Foot-and-mouth disease (FMD) is endemic in most countries in southern Africa. African buffaloes (Syncerus caffer) are known to play a significant role in the transmission and dynamics of FMD virus (FMDV) in wildlife-livestock interface areas. The aim of this study was to investigate the serotype and determine the genetic relationships of FMDV recovered from animals in Tanzania, Zambia and Mozambique, and compare them with viruses detected from elsewhere in the sub-Saharan region.